Most marine bacteria can form biofilms, and they are the main components of biofilms observed on marine surfaces. Biofilms constitute a widespread life strategy, as growing in such structures offers many important biological benefits. The molecular compounds expressed in biofilms and, more generally, the metabolomes of marine bacteria remain poorly studied. In this context, a nontargeted LC–MS metabolomics approach of marine biofilm-forming bacterial strains was developed. Four marine bacteria, Persicivirga (Nonlabens) mediterranea TC4 and TC7, Pseudoalteromonas lipolytica TC8, and Shewanella sp. TC11, were used as model organisms. The main objective was to search for some strain-specific bacterial metabolites and to determine how culture parameters (culture medium, growth phase, and mode of culture) may affect the cellular metabolism of each strain and thus the global interstrain metabolic discrimination. LC–MS profiling and statistical partial least-squares discriminant analyses showed that the four strains could be differentiated at the species level whatever the medium, the growth phase, or the mode of culture (planktonic vs biofilm). A MS/MS molecular network was subsequently built and allowed the identification of putative bacterial biomarkers. TC8 was discriminated by a series of ornithine lipids, while the P. mediterranea strains produced hydroxylated ornithine and glycine lipids. Among the P. mediterranea strains, TC7 extracts were distinguished by the occurrence of diamine derivatives, such as putrescine amides.
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2017
Articles
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Nouvelle sonde fluorescente sélective et sensible pour la détection des ions Pb2+
17 April 2018, by CaB -
Développement d’un système mésofluidique imprimé en 3D pour le dosage du plomb dans les eaux naturelles
17 April 2018, by CaB -
Discrimination of four marine biofilm-forming bacteria by LC-MS metabolomics and influence of culture parameters
31 March 2017 -
Etude des Interactions Amibes-Bactéries Marines et détection de molécules signal
20 October 2020Acronym :
ABAMAR Funding:
EMBRC-France Short Overview:
This project allowed the acces to the services and platform bellonging to the Oceanological observatory of Banyuls-sur-mer, en partenariat avec R. Lami, LBBM-USR Involved MAPIEM permanent members: MOLMERET MaëllePartners:LBBM-USR 3579
LAMI Raphaël -
Spatio-temporal variations of marine biofilm communities colonizing artificial substrata including antifouling coatings in contrasted coastal environments
1 December 2017 -
Properties of polymer composite materials based on epoxy resin incorporated BaTiO3/Graphene oxyde nanoparticules
21 September 2018, by LB -
Electroactive Polyacrylate for antifouling applications
1 February 2018 -
Polyaniline thermoset blends and composites
24 September 2018, by Hugues BRISSET -
Photo-oxidative degradation behavior of linseed oil based epoxy resin
20 January 2017, by Hugues BRISSET -
Validation d’un Volume Elémentaire Représentatif pour composites UD renforcés par des fibres : influence des interphases
17 July 2018, by YJ