Most marine bacteria can form biofilms, and they are the main components of biofilms observed on marine surfaces. Biofilms constitute a widespread life strategy, as growing in such structures offers many important biological benefits. The molecular compounds expressed in biofilms and, more generally, the metabolomes of marine bacteria remain poorly studied. In this context, a nontargeted LC–MS metabolomics approach of marine biofilm-forming bacterial strains was developed. Four marine bacteria, Persicivirga (Nonlabens) mediterranea TC4 and TC7, Pseudoalteromonas lipolytica TC8, and Shewanella sp. TC11, were used as model organisms. The main objective was to search for some strain-specific bacterial metabolites and to determine how culture parameters (culture medium, growth phase, and mode of culture) may affect the cellular metabolism of each strain and thus the global interstrain metabolic discrimination. LC–MS profiling and statistical partial least-squares discriminant analyses showed that the four strains could be differentiated at the species level whatever the medium, the growth phase, or the mode of culture (planktonic vs biofilm). A MS/MS molecular network was subsequently built and allowed the identification of putative bacterial biomarkers. TC8 was discriminated by a series of ornithine lipids, while the P. mediterranea strains produced hydroxylated ornithine and glycine lipids. Among the P. mediterranea strains, TC7 extracts were distinguished by the occurrence of diamine derivatives, such as putrescine amides.
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Articles
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Exploring the chemodiversity of tropical microalgae for the discovery of natural antifouling compounds
4 February 2019 -
Discrimination of four marine biofilm-forming bacteria by LC-MS metabolomics and influence of culture parameters
31 March 2017 -
3D-printed lab-on-valve for fluorescent determination of cadmium and lead in water
6 April 2018, by CaB -
Morphology and thermal properties of PLA–cellulose nanofibers composites
3 June 2016 -
Preparation and characterization of polyaniline in reversed micelles of decylphosphonic acid for active corrosion protection coatings
2 June 2016 -
Organic thin film transistors based on distyryl-oligothiophenes: Role of AFM images in analyses of charge transport properties
3 June 2016 -
Metabolomic profiling reveals deep chemical divergence between two morphotypes of the zoanthid Parazoanthus axinellae
2 June 2016 -
Degradation of rubber to metals bonds during its cathodic delamination, validation of an artificial ageing test
3 June 2016 -
Editorial: Impact and Management of Marine Biofouling
6 September 2022 -
An insight of enhanced natural material (calcined diatomite) efficiency in nickel and silver retention: Application to natural effluents
20 April 2020, by CaB
Laboratoire MAPIEM (UR 4323)