Most marine bacteria can form biofilms, and they are the main components of biofilms observed on marine surfaces. Biofilms constitute a widespread life strategy, as growing in such structures offers many important biological benefits. The molecular compounds expressed in biofilms and, more generally, the metabolomes of marine bacteria remain poorly studied. In this context, a nontargeted LC–MS metabolomics approach of marine biofilm-forming bacterial strains was developed. Four marine bacteria, Persicivirga (Nonlabens) mediterranea TC4 and TC7, Pseudoalteromonas lipolytica TC8, and Shewanella sp. TC11, were used as model organisms. The main objective was to search for some strain-specific bacterial metabolites and to determine how culture parameters (culture medium, growth phase, and mode of culture) may affect the cellular metabolism of each strain and thus the global interstrain metabolic discrimination. LC–MS profiling and statistical partial least-squares discriminant analyses showed that the four strains could be differentiated at the species level whatever the medium, the growth phase, or the mode of culture (planktonic vs biofilm). A MS/MS molecular network was subsequently built and allowed the identification of putative bacterial biomarkers. TC8 was discriminated by a series of ornithine lipids, while the P. mediterranea strains produced hydroxylated ornithine and glycine lipids. Among the P. mediterranea strains, TC7 extracts were distinguished by the occurrence of diamine derivatives, such as putrescine amides.
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MEBMB
Articles
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A comparative biomonitoring study of trace metals and organic compounds bioaccumulation in marine biofilms and caged mussels along the French Mediterranean coast
27 January, by JFB -
Discrimination of four marine biofilm-forming bacteria by LC-MS metabolomics and influence of culture parameters
31 March 2017 -
Stop alle Plastiche in H2O!
12 June 2019, by Hugues BRISSETAcronym:
SPLASH Funding:
[Programme MARITTIMO Interreg Italie-France Maritime] Short Overview:
SpLASH, via pluridisciplinary methodologies, aims to retrieve data on microplastics occurrence at the surface but also in the seawater column, the superficial sediments and biotic compartiments. In addition, like all surfaces immersed in marine environments, microplastics are quickly colonized by microorganisms that lead to 3D structures called biofilms. Their characteristics are associated (…) -
BARRE Abel
1 October 2021From 01/10/2021 to 30/09/2024 Research Topics
BIOFINDIC Project -
Characterization of the metabolic production of marine biofilms. Towards an application to the study of complex biofilms in situ
29 November 2018Beginning: November 2018
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Identification et caractérisation des exopolymères de biofilms de bactéries marines
9 June 2016, by Hugues BRISSETBeginning: 2010
Defence: February 6, 2014 -
THOLLET Simon
17 January, by Farah IBRAHIMFrom 07/01/2025 to 04/07/2025 Research Topics
Resistomes of coastal ecosystem biofilms: a metagenomic approach -
Clean Antifouling Coating (REVêtement Antifouling Propres)
3 July 2018, by Hugues BRISSETAcronym :
REVAP Funding:
Programme de soutien à la Recherche (PSR-SIIRI) du ministère Economie, Science et Innovation du Quebec Short Overview:
The aim of the REVAP project will be to assess the antifouling efficacy of a new type of coating based on xerogel and consequently environmentally friendly. This three years project, initiated by Pr Karine Lemarchand (UQAR/ISMER Rimouski, QC) will be achieved in collaboration with Dr. Jean-François Briand (UTLN, France) and the Mirapakon (…) -
PRIME Anne-Hélène
25 February 2020From 10/07/2018 to 30/08/2021
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What governs marine fouling assemblages on chemically-active antifouling coatings?
15 January 2022, by JFB