MEBMB

Articles

• Impact of phosphate starvation on the metabolome of the biofilm-forming marine bacterium Pseudoalteromonas lipolytica
  24 May 2022
• Discrimination of four marine biofilm-forming bacteria by LC-MS metabolomics and influence of culture parameters
  31 March 2017

  Most marine bacteria can form biofilms, and they are the main components of biofilms observed on marine surfaces. Biofilms constitute a widespread life strategy, as growing in such structures offers many important biological benefits. The molecular compounds expressed in biofilms and, more generally, the metabolomes of marine bacteria remain poorly studied. In this context, a nontargeted LC–MS metabolomics approach of marine biofilm-forming bacterial strains was developed. Four marine bacteria, Persicivirga (Nonlabens) mediterranea TC4 and TC7, Pseudoalteromonas lipolytica TC8, and Shewanella sp. TC11, were used as model organisms. The main objective was to search for some strain-specific bacterial metabolites and to determine how culture parameters (culture medium, growth phase, and mode of culture) may affect the cellular metabolism of each strain and thus the global interstrain metabolic discrimination. LC–MS profiling and statistical partial least-squares discriminant analyses showed that the four strains could be differentiated at the species level whatever the medium, the growth phase, or the mode of culture (planktonic vs biofilm). A MS/MS molecular network was subsequently built and allowed the identification of putative bacterial biomarkers. TC8 was discriminated by a series of ornithine lipids, while the P. mediterranea strains produced hydroxylated ornithine and glycine lipids. Among the P. mediterranea strains, TC7 extracts were distinguished by the occurrence of diamine derivatives, such as putrescine amides.

• Towards improving biofouling knowledge within MRE environments
  25 April 2024, by ChB
• Biofouling: the need to develop interdisciplinary teamwork skills for MRE
  25 April 2024, by ChB
• What governs marine fouling assemblages on chemically-active antifouling coatings?
  15 January 2022, by JFB
• Environmental microbiome diversity and stability is a barrier to antimicrobial resistance gene accumulation
  3 September 2024, by EC
• Metabolomic fingerprinting of 8 mangroves species from North Vietnam
  16 January 2018
• PORTAS Aurélie
  5 September 2023

  From 01/09/2023 to 31/08/2024

• LC-MS based metabolic profiling of marine bacterial strains demonstrates variation between free-living and biofilm lifestyles
  12 July 2016
• Electroactive Polyacrylate for antifouling applications
  1 February 2018