ORTALO-MAGNE Annick

Articles

• Impact of hydrodynamics on community structure and metabolic production of marine biofouling formed in a highly energetic estuary
  25 March 2024, by JFB
• Metabolomic and proteomic changes induced by growth inhibitory concentrations of copper in the biofilm-forming marine bacterium Pseudoalteromonas lipolytica
  23 September 2019
• Caractérisation par analyse métabolomique de biomarqueurs bactériens au sein de biofilms marins
  6 June 2016
• Effet de l’hydrodynamisme sur la production biochimique de biofilms marins
  25 August 2023, by JFB
• Discrimination of four marine biofilm-forming bacteria by LC-MS metabolomics and influence of culture parameters
  31 March 2017

  Most marine bacteria can form biofilms, and they are the main components of biofilms observed on marine surfaces. Biofilms constitute a widespread life strategy, as growing in such structures offers many important biological benefits. The molecular compounds expressed in biofilms and, more generally, the metabolomes of marine bacteria remain poorly studied. In this context, a nontargeted LC–MS metabolomics approach of marine biofilm-forming bacterial strains was developed. Four marine bacteria, Persicivirga (Nonlabens) mediterranea TC4 and TC7, Pseudoalteromonas lipolytica TC8, and Shewanella sp. TC11, were used as model organisms. The main objective was to search for some strain-specific bacterial metabolites and to determine how culture parameters (culture medium, growth phase, and mode of culture) may affect the cellular metabolism of each strain and thus the global interstrain metabolic discrimination. LC–MS profiling and statistical partial least-squares discriminant analyses showed that the four strains could be differentiated at the species level whatever the medium, the growth phase, or the mode of culture (planktonic vs biofilm). A MS/MS molecular network was subsequently built and allowed the identification of putative bacterial biomarkers. TC8 was discriminated by a series of ornithine lipids, while the P. mediterranea strains produced hydroxylated ornithine and glycine lipids. Among the P. mediterranea strains, TC7 extracts were distinguished by the occurrence of diamine derivatives, such as putrescine amides.

• Caractérisation et activité anti-biofilm des exopolysaccharides de la bactérie marine Pseudoalteromonas ulvae TC14
  15 September 2016
• LC-MS based metabolic profiling of marine bacterial strains demonstrates variation between free-living and biofilm lifestyles
  12 July 2016
• Characterization of the metabolic production of marine biofilms. Towards an application to the study of complex biofilms in situ
  29 November 2018

  Beginning: November 2018

• Impact of phosphate starvation on the metabolome of the biofilm-forming marine bacterium Pseudoalteromonas lipolytica
  24 May 2022
• Recherche de biomarqueurs par analyse métabolomique à partir de bactéries issues de biofilms marins
  6 June 2016