Publication

Articles

• Bioinspiration and microtopography as non-toxic strategies for marine bioadhesion control
  14 December 2021, by JFB
• Antifouling activity of commercial biocides vs natural and natural-derived products assessed by marine bacteria adhesion bioassay
  3 June 2016
• Antifouling Activities of Immobilized Ferrocenyl Glucose on a Glassy Carbon Surface
  4 February 2022, by RB
• Influence of sharklet-inspired micropatterned polymers on spatio-temporal variations of marine biofouling
  5 December 2022, by JFB
• Aging effects at room temperature and process parameters on 3D-printed poly (lactic acid) (PLA) tensile properties
  16 April 2024, by IM
• Metabolome and proteome changes between biofilm and planktonic phenotypes of the marine bacterium Pseudoalteromonas lipolytica TC8
  16 January 2018
• Preparation and study of a carbohydrated carbon surface by electrochemical oxidation of beta-D-(4-aminophenoxy)glucopyranose
  19 September 2018, by ChB
• Long-lasting biofouling formation on transparent fouling-release coatings for the construction of efficient closed photobioreactors
  24 April 2024, by ChB
• Impact of phosphate starvation on the metabolome of the biofilm-forming marine bacterium Pseudoalteromonas lipolytica
  24 May 2022
• Discrimination of four marine biofilm-forming bacteria by LC-MS metabolomics and influence of culture parameters
  31 March 2017

  Most marine bacteria can form biofilms, and they are the main components of biofilms observed on marine surfaces. Biofilms constitute a widespread life strategy, as growing in such structures offers many important biological benefits. The molecular compounds expressed in biofilms and, more generally, the metabolomes of marine bacteria remain poorly studied. In this context, a nontargeted LC–MS metabolomics approach of marine biofilm-forming bacterial strains was developed. Four marine bacteria, Persicivirga (Nonlabens) mediterranea TC4 and TC7, Pseudoalteromonas lipolytica TC8, and Shewanella sp. TC11, were used as model organisms. The main objective was to search for some strain-specific bacterial metabolites and to determine how culture parameters (culture medium, growth phase, and mode of culture) may affect the cellular metabolism of each strain and thus the global interstrain metabolic discrimination. LC–MS profiling and statistical partial least-squares discriminant analyses showed that the four strains could be differentiated at the species level whatever the medium, the growth phase, or the mode of culture (planktonic vs biofilm). A MS/MS molecular network was subsequently built and allowed the identification of putative bacterial biomarkers. TC8 was discriminated by a series of ornithine lipids, while the P. mediterranea strains produced hydroxylated ornithine and glycine lipids. Among the P. mediterranea strains, TC7 extracts were distinguished by the occurrence of diamine derivatives, such as putrescine amides.